All the solutions were prepared in 100 mm phosphate buffer ph 7. Scavenging activity on dpph radical quantitative fig. In order to investigate the antioxidant activity, different complementary test systems, namely, 2,2diphenyl1picrylhydrazil dpph free radical scavenging, total phenolic, total tannin and. The compound sodium nitroprusside is known to decompose in aqueous solution at physiological ph 7. A comparative study on the antioxidant activity of methanolic. We measured the superoxide radical scavenging activity according to some. Antioxidant and free radical scavenging activity of spondias pinnata. Pdf hydrogen peroxide radical scavenging and total. Dpph free radical scavenging assay11 dpph 1,1diphenyl2picrylhydrazyl radical scavenging assay is a stable free radical with purple color, the intensity of which is measured at 510 nm spectrophotometrically. Radical scavenging and antioxidant activity of tannic acid. A more appropriate format for the assay is a decolorization technique in that the radical is.
A voucher specimen is available in the herbarium file of our department. Dose dependent radical scavenging activity was observed and the results were found to be comparable to that of. Studies on phytochemicals, antioxidant, free radical. The inhibitory effect of the chitosan on superoxide anion radical was ranging from 15. Superoxide anion scavenging activity of ethanolic extract of f. The superoxide radical scavenging activity was analyzed according to the procedure of.
The present study evaluated the in vitro antioxidant and antibacterial activities of various extracts of aerial parts of periploca aphylla and ricinus communis. Antioxidant enzymes and dpphradical scavenging activity. We show that increased cat, apx, gr, and dpphradical scavenging activity and protection of cat activity during chilling are correlated with heat shock. The crude aqueous extract of the plant contains the phenolics and flavonoids which are said to be the potent antioxidants 11. Hydroxyl radicals scavenging activity of chitosan 0. Measurement of superoxide anion scavenging activity was performed based on the method described by winterbourne et al 186. The superoxide radicalscavenging activity linear proportionally damped from 0. Radical scavenging property of a novel peptide derived from c. The antioxidant activity using the dpph 1, 1diphenyl2picrylhydrazyl assay was assessed by the method of blois8. Radical scavenging and antioxidant activity of carthamus tinctorius. The following free radical scavenging assays were carried out.
Antihypertensive, antioxidant and free radical scavenging. Data obtained showed that the extract scavenged superoxide anion radicals ic 501602 gml in a dose dependant manner using. In vitro antioxidant activities of the plant extract were determined by dpph and no scavenging method. Antioxidant activity assays superoxide radicalscavenging activity by nbt method superoxide radicals were generated by a modified method of beauchamp and fridovich 1971, described by zhishen, mengcheng, and jianming 1999. It is evident from the data that periploca aphylla and ricinus communis possess hydrogen donating capabilities and. Invitro antioxidant and free radical scavenging activity.
Transcript levels of antioxidative genes and oxygen radical. The objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s. Pdf hydroxyl radical scavenging activity of flavonoids. Superoxide anion radical scavenging activities of herbs and. The reactive oxygen ion superoxide is particularly important as the product of the oneelectron reduction of dioxygen o 2, which occurs widely in nature. Free radical scavenging activity of crude extracts and 4. The plant extracts exhibited strong antioxidant and radical scavenging activity on abts radical cation, dpph free radical, hydrogen peroxide. Superoxide radical reduced nbt to a bluecolored formation that was measured at 560 nm. The hydrogen peroxide scavenging assay was carried out following the procedure of ruch et al. Free radical scavenging activity of ethanolic extract of.
Caglarirmak 2007 reported that the nutritional value of l. In the present paper, we examines the in vitro radical scavenging and antioxidant capacity of tannic acid. In this study, the influencing factors have been systematically investigated for the first time, and a number of experiments have proved that the ph is. Superoxide radical scavenging properties of extracts and. Antioxidant, antimicrobial, and free radical scavenging. Dpph free radical scavenging activity of the extracts of the. Dpph free radical scavenging activity of the extracts of. In vitro nitric oxide scavenging activity of methanol. Radical scavenging activity of phenolics and flavonoids in.
Antioxidant and free radical scavenging activity of. A superoxide is a compound that contains the superoxide ion, which has the chemical formula o. Invitro antioxidant and free radical scavenging activity of. The antioxidant activity of the tannic acid, bha, bht. The superoxide scavenging activity of ethanolic extract of d. Assay of nitric oxide scavenging activity nitric oxide scavenging activity can be estimated by the use of griess iliosvoy reaction garrat, 1964. The extract was examined for different reactive oxygen species ros scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen. Antioxidant capacity and radioprotective properties of the. The preferred radical scavenging mechanisms of fisetin and. Maximum value of dpph radical scavenging activity of the water fraction of lpe was achieved after 3 days of fermentation, and then declined, which was associated with reduced phenolic content. Effect of chilling on antioxidant enzymes and dpphradical.
Superoxide radical scavenging activity was done by the nbt. Hydroxyl radical scavenging activity hrsa of the extractives was determined by the method of klein et al. Enhancing antioxidant, antiproliferation, and free radical. Antioxidant activities of extracts from five edible. Superoxide anion radical scavenging activity superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Investigation of the antioxidant activity of chitooligosaccharides on. Evaluation of free radical scavenging activity of an. Evaluation of abelmoschus moschatus extracts for antioxidant.
Free radical scavenging and antioxidant activities of. Total antioxidant activity, metal chelation, radical scavenging dpph effects and reducing power as well as activities destructive to active oxygen species such as the superoxide anion radical, hydroxyl radical, and hydrogen peroxide are widely used for this purpose shimada et al. Synthesis, characterization, and the antioxidant activity of. In vitro antioxidant and free radical scavenging activities. The research reported in this article was conducted to see if large differences in seedling vigour had an effect on the activity of selected antioxidant enzymes and dpph radical scavenging activity, and chilling tolerance in cucumber. Ttc activity is associated with nad dehydrogenase activity during aerobic respiration of roots shimada 1969. Molecular oxygen dioxygen is a diradical containing two unpaired electrons, and. In vitro antioxidant and free radical scavenging activity 39 roots are the main portions of the whole plant as they possess wide number of the therapeutic agents.
Free radical scavenging activities of watersoluble extracts from some natural publisher pdf plugin 2003 sources. Scavenging activities of these plants are comparable with standards. Free radical scavenging activities of watersoluble extracts from some natural sources. The antioxidant activity of the memq was evaluated by the. In addition, the nontoxic nature of the peptide was con. As positive controls, epicatechin and lascorbic acid were also examined for dpph radical scavenging activity. Free radical scavenging activity of ethanolic and acetone extract of alpinia galanga in order to determine the free radical scavenging activity of ethanolic and acetone extract of alpinia galanga, the following parameters were assayed. The original pyrogallol 1,2,3trihydroxybenzene method, which was developed specifically for superoxide dismutase, is now widely used for measuring superoxidescavenging of other antioxidants.
Transcript levels of aox increased substantially between 3 and 6 days in all. Radical scavenging and antioxidant activity of ethanolic. The free radical scavenging activities of these compounds were tested by their ability to bleach the stable radical dpph. Oh scavenging activities of pt nps experiments to determine the rate constant of the. Superoxide anion radical scavenging activity the superoxide anion scavenging activity of eess. A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples. Assay for superoxide radical scavenging activity was based on the capacity of the sample to inhibit blue formazan formation by scavenging the superoxide radicals generated in riboflavinlightnbt system 5,10,12. Free radical scavenging activity of the methanol extract was tested in three in vitro models, viz.
Superoxide anion radical scavenging activities of herbs. Antioxidant enzymes and dpphradical scavenging activity in. The extract was examined for different reactive oxygen species ros scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and. Copperii complexes showed appreciable superoxide radical scavenging activity with ic 50 values ranging from 1. Moreover, determination of intracellular ros level was performed to con. The research reported in this article was conducted to see if large differences in seedling vigour had an effect on the activity of selected antioxidant enzymes and dpphradical scavenging activity, and chilling tolerance in cucumber. The original pyrogallol 1,2,3trihydroxybenzene method, which was developed specifically for superoxide dismutase, is now widely used for measuring superoxide scavenging of other antioxidants. The freeradical scavenging activities of these compounds were tested by their ability to bleach the stable radical dpph.
Antioxidant activity assays superoxide radical scavenging activity by nbt method superoxide radicals were generated by a modified method of beauchamp and fridovich 1971, described by zhishen, mengcheng, and jianming 1999. Superoxide anion radical scavenging activity biology essay. Determination of superoxide radical scavenging activity superoxide radical scavenging activity of eseta was measured by the reduction of nitro blue tetrazolium nbt according to our previously reported method. The radical scavenging activity, antioxidant activity by carotene bleaching assay and, superoxide dismutase sod like ability decreased in the order of 70% methanol, 70% ethanol, cm, ea and, n.
The antioxidant activity of the aerial part extract of m. Radical scavenging property of a novel peptide derived. Oh reaction hav e been reported previously, using both a fenton reaction system 6, and a uvh 2o2 system as an advanced oxidization process aop for attaining the oxidative degradation of organic pollutants 7. Aug 17, 2011 superoxide radical scavenging activity. The hydroxyl radicalscavenging assay was done by the method of. Three extracts of seed amsi, amsii and amsiii and one leaf extract amli were found to be an efficient scavenger of superoxide radical generation. A comparative study on the antioxidant activity of. The total phenolics and flavonoid content and dpph and superoxide radical scavenging activity in leaf extract of catharanthus roseus, mentha arvensis, murraya. Antioxidant and free radical scavenging activity of spondias. Superoxide generated by pyrogallol reduces highly watersoluble tetrazolium salt to produce a soluble formazan. Superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Table 1 shows the percentage of dpph radical scavenged by standards, that is, npropyl gallate and 3tbutyl4hydroxyanisole and different extracts of 2 plants at various concentrations.
Antioxidant activities of extracts from five edible mushrooms. The ic50 values for scavenging of free radicals like for hydroxyl, superoxide, nitric oxide, hydrogen peroxide, singlet oxygen and hypochlorous acid by mb, ab. In addition to that ascorbic acid and butylated hydroxyl toluene were used as the reference antioxidant. Free radical scavenging activity, total phenolic content. A further molecular docking technique was employed to understand the binding of the complexes toward the molecular target dna and human dna topoisomerase i. Chrysin, rutin and quercetin were run to explore the effect of. Antioxidant and free radical scavenging activity of pithecellobium. The corresponding oxygen radical scavenging enzyme activities including sod, apx, cat, and peroxidise pod in treated samples were also higher than those in the control for the. The antioxidant activity of the aqueous ethanolic extract of doum leaves, hyphaene thebaica l. The antioxidantradical reaction was initiated by addition of xanthine oxidase solution xod, whereas 5tertbutoxycarbonyl 5methyl1.
The order of greatest hydroxyl radical scavenging activity was green tea. Pdf antioxidant and free radical scavenging activity of curcumin. Analysis of gamma rayinduced radicals and radicalscavenging. The reaction mixture contained 50 mm phosphate buffer ph 7. As shown in table 1, a great change in the dpph radical scavenging activity occurred after fermentation with a. In vitro evaluation of free radical scavenging activity of. Transcript levels of antioxidative genes and oxygen. Superoxide anion radical activity was measured by the. Dnaprotein binding, dna cleavage, cytotoxicity, superoxide.